Potter Huntington
Harvard Medical School, Boston, Massachusetts, USA.
Curr Protoc Mol Biol. 2003 May;Chapter 9:Unit 9.3. doi: 10.1002/0471142727.mb0903s62.
Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. In this unit, the describes the electroporation of mammalian cells and an outlines modifications for preparation and transfection of plant protoplasts.
电穿孔法——利用高压电击将DNA导入细胞——可用于大多数细胞类型，能产生高频率的稳定转化和瞬时基因表达，而且由于所需步骤较少，可能比其他技术更简便。在本单元中，描述了哺乳动物细胞的电穿孔法，并概述了植物原生质体制备和转染的改良方法。
