牛疱疹病毒4对大鼠脑的转导

Transduction of the rat brain by Bovine Herpesvirus 4.

作者信息

Redaelli Marco, Cavaggioni Andrea, Mucignat-Caretta Carla, Cavirani Sandro, Caretta Antonio, Donofrio Gaetano

机构信息

Department of Human Anatomy and Physiology, University of Padova, 35131 Padova, Italy.

出版信息

Genet Vaccines Ther. 2008 Feb 12;6:6. doi: 10.1186/1479-0556-6-6.

DOI:10.1186/1479-0556-6-6

PMID:18267037

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2259350/

Abstract

Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFP Delta TK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFP Delta TK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.

摘要

牛疱疹病毒4型（BoHV - 4）是一种γ疱疹病毒，与明确的疾病关联不明显。一种表达绿色荧光蛋白（EGFP）的重组牛疱疹病毒4型（BoHV - 4EGFP ΔTK）已成功用于感染F98大鼠胶质瘤细胞。将BoHV - 4EGFP ΔTK注入大鼠脑侧脑室。组织学和免疫组织化学显示室管膜细胞和吻侧迁移流细胞被转导，而神经元未被转导。对90天的临床评分表明该病毒无神经致病性，提示这种病毒是脑肿瘤基因治疗的合适载体。