Osorio F A, Rock D L, Reed D E
J Gen Virol. 1985 Sep;66 ( Pt 9):1941-51. doi: 10.1099/0022-1317-66-9-1941.
Herpesvirus Movar 33/63 is the prototype strain of a group of slow growth bovine herpesviruses which have been reported to exhibit cytomegalovirus-like characteristics. These viruses have the ability to produce long-term persistent infections of spleen and other lymphoreticular organs in both cattle and rabbits. Rabbits were inoculated with a suspension of Movar 33/63 propagated in cell culture, and sacrificed at intervals between 3 days and 49 weeks post-infection. Cell-free infectious virus was detected only in conjunctival secretions, buffy coat and spleen homogenates up to 7 days post-infection. Beyond this brief acute replication period, co-cultivation or explantation was required for the detection of viral infectivity. The spleen, the only organ from which virus was consistently recovered, exhibited the highest infectious titres as detected by infectious centre assay. The use of several cell isolation techniques (including solid-phase fractionation on ligand-coated surfaces, nylon wool filtration, affinity chromatography, immunocytolysis and plastic surface adherence) allowed separation of B-enriched, T-enriched and non-T, non-B cell fractions. Infectivity during the acute and persistent phase of the infection was associated with the non-T, non-B population which was highly enriched in adherent and non-adherent spleen mononuclear phagocytes. No virus was isolated from either T or B cells.
疱疹病毒Movar 33/63是一组生长缓慢的牛疱疹病毒的原型毒株，据报道其具有类似巨细胞病毒的特征。这些病毒能够在牛和兔子的脾脏及其他淋巴网状器官中产生长期持续性感染。用在细胞培养中增殖的Movar 33/63悬液接种兔子，并在感染后3天至49周的不同时间点处死兔子。在感染后7天内，仅在结膜分泌物、血沉棕黄层和脾脏匀浆中检测到无细胞感染性病毒。在这个短暂的急性复制期之后，需要通过共培养或外植法来检测病毒感染性。脾脏是唯一持续能分离出病毒的器官，通过感染中心试验检测，其具有最高的感染滴度。使用多种细胞分离技术（包括在配体包被表面上的固相分级分离、尼龙毛过滤、亲和层析、免疫细胞溶解和塑料表面黏附）可分离出富含B细胞、富含T细胞以及非T、非B细胞组分。在感染的急性期和持续期，感染性与非T、非B细胞群体相关，该群体在贴壁和非贴壁脾脏单核吞噬细胞中高度富集。未从T细胞或B细胞中分离到病毒。
