Osorio F A, Reed D E, Rock D L
Vet Microbiol. 1982 Dec;7(6):503-13. doi: 10.1016/0378-1135(82)90045-1.
A strain of bovine herpesvirus-4 (BHV-4) isolated from bovine cases of mammary pustular dermatitis was used for experimental infection of rabbits. The strain is serologically indistinguishable from the group prototype Movar 33/63 and from the American isolate DN599. Groups of rabbits were inoculated by various routes. Intravaginal and conjunctival inoculations resulted in vulvovaginitis and conjunctivitis, respectively, and in shedding of virus. The rabbits seroconverted for the virus, with high titers of antibodies (indirect fluorescent antibody test) that persisted throughout the experiment. Treatment with dexamethasone, beyond the acute infection, did not produce recrudescence of disease or shedding of the virus. Rabbits were killed at various times, from 3 to 6 months post-infection, and the virus was recovered from explant cultures of spleen and by cocultivation of spleen cells with bovine lung cells. These results demonstrate the usefulness of the rabbit as a model for studying the pathogenesis of BHV-4 infection in cattle.
从牛乳房脓疱性皮炎病例中分离出的一株牛疱疹病毒4型（BHV-4）用于对家兔进行实验性感染。该毒株在血清学上与该组原型毒株Movar 33/63以及美国分离株DN599无法区分。将家兔分组并通过不同途径接种。经阴道接种和结膜接种分别导致外阴炎和阴道炎以及结膜炎，并出现病毒脱落。家兔针对该病毒发生血清转化，产生了高滴度抗体（间接荧光抗体试验），且在整个实验过程中持续存在。在急性感染期过后，用地塞米松治疗并未导致疾病复发或病毒脱落。在感染后3至6个月的不同时间点处死家兔，并从脾脏外植体培养物以及通过将脾细胞与牛肺细胞共培养来回收病毒。这些结果证明家兔作为研究牛BHV-4感染发病机制模型的有用性。
