Wang Yu-wen, Li Jian-hong, Wu Min, Wang Yi-yu, Weng Yong-ping
Life Sciences College, Nanjing Normal University, Nanjing 210097, China.
Huan Jing Ke Xue. 2007 Oct;28(10):2187-91.
The aim of the present study was to clarify the community composition of cyanobacterial bloom in Xuanwu Lake of Nanjing city. The Microcystis colonies in cyanobacterial blooms sampled from Xuanwu Lake during August 2005 and September 2006 were identified by morphological characteristics and internal transcribed spacer sequence (ITS). The results indicated that the bloom was composed mainly by Microcystis aeruginosa, M. novacekii and M. wesenbergii. The biomass percentage of them was 30%-45%, 35%-40% and 10%-15%, respectively. ITS of two dominant Microcystis strains were amplified by PCR and sequenced to identify their species. The analysis of ITS sequences and the ITS phylogenetic tree demonstrated that the two dominant species should be M. aeruginosa and M. novacekii. A microcystin-producing gene mcyB was detected to test toxic strain. A unique product of approximately 780 bp was amplified in two dominant strains. It indicated that both of them were of microcystin-producing genotype. High performance liquid chromatography (HPLC) analysis showed that the toxins in the native bloom biomass were mainly MC-LR and MC-RR.
本研究的目的是阐明南京市玄武湖蓝藻水华的群落组成。通过形态特征和内转录间隔区序列（ITS）对2005年8月至2006年9月从玄武湖采集的蓝藻水华中的微囊藻菌落进行鉴定。结果表明，水华主要由铜绿微囊藻、新微囊藻和惠氏微囊藻组成。它们的生物量百分比分别为30%-45%、35%-40%和10%-15%。通过PCR扩增两种优势微囊藻菌株的ITS并进行测序以鉴定其种类。ITS序列分析和ITS系统发育树表明，两种优势种应为铜绿微囊藻和新微囊藻。检测微囊藻毒素产生基因mcyB以测试有毒菌株。在两种优势菌株中扩增出约780 bp的独特产物。这表明它们均为微囊藻毒素产生基因型。高效液相色谱（HPLC）分析表明，天然水华生物量中的毒素主要为MC-LR和MC-RR。
