Wu Yi-Hsuan, Tseng Ching-Ping, Cheng Mei-Ling, Ho Hung-Yao, Shih Shin-Ru, Chiu Daniel Tsun-Yee
Graduate Institute of Basic Medical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
J Infect Dis. 2008 Mar 15;197(6):812-6. doi: 10.1086/528377.
The host cellular environment is a key determinant of pathogen infectivity. Viral gene expression and viral particle production of glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD-knockdown cells were much higher than their counterparts when human coronavirus (HCoV) 229E was applied at 0.1 multiplicity of infection. These phenomena were correlated with increased oxidant production. Accordingly, ectopic expression of G6PD in G6PD-deficient cells or addition of antioxidant (such as alpha-lipoic acid) to G6PD-knockdown cells attenuated the increased susceptibility to HCoV 229E infection. All experimental data indicated that oxidative stress in host cells is an important factor in HCoV 229E infectivity.
宿主细胞环境是病原体感染性的关键决定因素。当以0.1感染复数应用人冠状病毒(HCoV)229E时,葡萄糖-6-磷酸脱氢酶(G6PD)缺陷型和G6PD敲低型细胞的病毒基因表达和病毒颗粒产生比其对照细胞高得多。这些现象与氧化剂产生增加相关。因此,在G6PD缺陷型细胞中异位表达G6PD或向G6PD敲低型细胞中添加抗氧化剂(如α-硫辛酸)可减弱对HCoV 229E感染增加的易感性。所有实验数据表明,宿主细胞中的氧化应激是HCoV 229E感染性的一个重要因素。