Fujita M, Iida H, Asaka M, Izumino K, Takata M, Sasayama S
Second Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Nephron. 1991;57(2):201-5. doi: 10.1159/000186251.
Effect of the immunosuppressive agent, ciclosporin (CS), on bovine serum albumin (BSA) nephritis in rats was evaluated. Eight weeks after immunization, 19 male Wistar rats received a daily intravenous dose of BSA (2 mg). Two weeks later, 11 rats received BSA and an oral dose of CS (10 mg/kg), and 8 rats received only BSA for 2 weeks. Urinary protein was measured weekly and serum anti-BSA antibody was measured by passive hemagglutination biweekly. The animals were killed at the 12th experimental week and blood samples and kidney specimens were obtained. BUN and serum creatinine were measured at the time of sacrifice. Kidney specimens were processed for light and immunofluorescent microscopic examination. Urinary protein excretion was significantly less in CS-treated rats than in nontreated controls at the 2nd week after treatment (5.3 +/- 1.3 vs. 25.6 +/- 10.3 mg/day, p less than 0.05). Anti-BSA antibody titers were lower in treated rats than in controls at the 2nd week after the treatment. There were no significant differences in the levels of BUN and serum creatinine between two groups. Glomerular hypercellularity and mesangial widening were milder in treated rats than in controls, and glomerular deposition of BSA was less intense in treated rats than in controls. These results suggest that CS suppressed the antibody production and the development of glomerular changes in rats with immune complex glomerulonephritis.
评估免疫抑制剂环孢素（CS）对大鼠牛血清白蛋白（BSA）肾炎的影响。免疫8周后，19只雄性Wistar大鼠每日静脉注射BSA（2毫克）。两周后，11只大鼠接受BSA并口服CS（10毫克/千克），8只大鼠仅接受BSA，持续2周。每周测量尿蛋白，每两周通过被动血凝法测量血清抗BSA抗体。在实验第12周处死动物，采集血样和肾脏标本。处死时测量血尿素氮和血清肌酐。对肾脏标本进行光镜和免疫荧光显微镜检查。治疗后第2周，CS治疗组大鼠的尿蛋白排泄明显低于未治疗对照组（5.3±1.3对25.6±10.3毫克/天，p<0.05）。治疗后第2周，治疗组大鼠的抗BSA抗体滴度低于对照组。两组之间血尿素氮和血清肌酐水平无显著差异。治疗组大鼠的肾小球细胞增多和系膜增宽比对照组轻，治疗组大鼠的肾小球BSA沉积比对照组弱。这些结果表明，CS抑制了免疫复合物性肾小球肾炎大鼠的抗体产生和肾小球病变的发展。
