Yamamoto T, Kihara I, Morita T, Oite T
Acta Pathol Jpn. 1978 Nov;28(6):859-66. doi: 10.1111/j.1440-1827.1978.tb01276.x.
Proliferative glomerulonephritis with proteinuria was induced in Wistar rats by bovine serum albumin (BSA). Rats were first immunized with 1 mg or 2.5 mg of BSA and complete Freund's adjuvant (CFA) and eight weeks later 1 mg of BSA were given intravenously six times a week for four weeks. Immunofluorescence revealed granular deposits of IgG, C3, and BSA in the mesangial area with or without deposition of the same components along the capillary wall. Evaluation of the circulating antibody disclosed an apparent correlation between the level of antibody and histological findings. Rats with an intermediate amount of antibody production developed mesangial widening with mesangial immune deposits and no proteinuria. Rats with a low response developed proliferative glomerulonephritis with immune deposits along the capillary wall as well as in the mesangial area and proteinuria.
通过牛血清白蛋白（BSA）在Wistar大鼠中诱导出伴有蛋白尿的增殖性肾小球肾炎。大鼠首先用1毫克或2.5毫克BSA和完全弗氏佐剂（CFA）进行免疫，8周后每周静脉注射1毫克BSA，共6次，持续4周。免疫荧光显示，在系膜区有IgG、C3和BSA的颗粒状沉积，沿毛细血管壁可有或无相同成分的沉积。对循环抗体的评估显示，抗体水平与组织学结果之间存在明显相关性。抗体产生量中等的大鼠出现系膜增宽，伴有系膜免疫沉积物，但无蛋白尿。抗体反应低的大鼠出现增殖性肾小球肾炎，在毛细血管壁以及系膜区有免疫沉积物，并伴有蛋白尿。
