Goddeeris B M, Dunlap S, Bensaid A, MacHugh N D, Morrison W I
ILRAD, Nairobi, Kenya.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):195-9. doi: 10.1016/0165-2427(91)90100-q.
Monoclonal antibodies specific for T cell differentiation antigens were tested on four cloned populations of lymphocytes derived from the peripheral blood mononuclear cells of an animal immunised with Theileria parva. The clones were defined functionally in terms of cytotoxic activity, MHC restriction and expression of messenger RNA for CD3 and T cell receptor (TCR). Two clones contained RNA transcripts for CD3, TCR-alpha and beta and were positive for CD2, CD5 and CD6; one of these was a typical CD4+ class II MHC-restricted non-cytotoxic clone while the other was a CD8+ class I MHC-restricted cytotoxic clone. By contrast, the remaining two clones had the characteristics of non-specific killer cells in that they exhibited moderate levels of non-MHC-restricted killing; they contained TCR-delta mRNA and a 1.2 kb truncated form of TCR-beta message, but they did not contain CD3 or TCR-alpha mRNA. One of these non-specific killer clones only expressed CD2 whereas the other clone only expressed CD8, but without the CD8 determinant recognised by monoclonal antibodies CC58 and BAT52. All four clones were negative for the WC1 antigen which is expressed on gamma/delta T lymphocytes.
针对T细胞分化抗原的单克隆抗体在四个克隆的淋巴细胞群体上进行了测试，这些淋巴细胞群体来源于用小泰勒虫免疫的动物的外周血单个核细胞。根据细胞毒性活性、MHC限制以及CD3和T细胞受体（TCR）信使RNA的表达，对这些克隆进行了功能定义。两个克隆含有CD3、TCR-α和β的RNA转录本，并且CD2、CD5和CD6呈阳性；其中一个是典型的CD4+II类MHC限制的非细胞毒性克隆，而另一个是CD8+I类MHC限制的细胞毒性克隆。相比之下，其余两个克隆具有非特异性杀伤细胞的特征，即它们表现出中等水平的非MHC限制杀伤；它们含有TCR-δ mRNA和1.2 kb截短形式的TCR-β信使，但不含有CD3或TCR-α mRNA。这些非特异性杀伤克隆中的一个仅表达CD2，而另一个克隆仅表达CD8，但不表达单克隆抗体CC58和BAT52识别的CD8决定簇。所有四个克隆的WC1抗原均为阴性，WC1抗原在γ/δ T淋巴细胞上表达。
