Yang Ling-Ling, Zhi Xiao-Yang, Li Wen-Jun
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China.
Wei Sheng Wu Xue Bao. 2007 Dec;47(6):951-5.
In order to understand the phylogenetic relationships among Nocardiopsis species more rationally, the gyrB, sod and rpoB gene partial sequences of 24 validly published Nocardiopsis species were determined. The phylogenetic trees were reconstructed including 16S rRNA gene and above-mentioned three housekeeping genes. The average similarities of gyrB, sod and rpoB of Nocardiopsis species were 87.7%, 87.3% and 94.1%, respectively. Meanwhile, the average similarity of 16S rRNA gene of Nocardiopsis species was 96.65%. The variabilities of gyrB, sod and rpoB gene in Nocardiospis are greater than that of 16S rRNA gene. By comparing the phylogenetic trees, the topology of gyrB gene tree is almost consistent with that of 16S rRNA gene tree. Consequently, the gyrB gene possesses the superiority in phylogenetic taxonomy of the genus Nocardiopsis.
为了更合理地了解诺卡氏菌属物种之间的系统发育关系，测定了24个已有效发表的诺卡氏菌属物种的gyrB、sod和rpoB基因部分序列。重建了包括16S rRNA基因和上述三个管家基因的系统发育树。诺卡氏菌属物种的gyrB、sod和rpoB的平均相似性分别为87.7%、87.3%和94.1%。同时，诺卡氏菌属物种的16S rRNA基因的平均相似性为96.65%。诺卡氏菌中gyrB、sod和rpoB基因的变异性大于16S rRNA基因。通过比较系统发育树，gyrB基因树的拓扑结构与16S rRNA基因树的拓扑结构几乎一致。因此，gyrB基因在诺卡氏菌属的系统发育分类中具有优势。
