使用高效毛细管电泳法同时测定饲料中的五种苯并咪唑类药物。
Simultaneous determination of five benzimidazoles in feeds using high-performance capillary electrophoresis.
作者信息
Shen Jianzhong, Tong Jing, Jiang Haiyang, Rao Qinxiong, Li Na, Guo Liming, Ding Shuangyang
机构信息
China Agricultural University, National Reference Laboratories for Veterinary Drug Residue, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100193, People's Republic of China.
出版信息
J AOAC Int. 2009 Jul-Aug;92(4):1009-15.
A high-performance capillary electrophoretic method for determination of five benzimidazoles (thiabendazole, albendazole, fenbendazole, mebendazole, oxfendazole) in animal feeds was developed. Separation was carried out in an 80.5 cm x 75 microm id fused-silica uncoated capillary and injected by hydrodynamic injection (35 mbar x 8 s) at a wavelength detection of 295 nm. The drugs were completely resolved in 50 mmol/L citric acid buffer (pH 1.9) with 30 kV at a temperature of 25 degrees C. Linear range was 1-80 microg/mL; limits of detection for the analytes were less than 2 mg/kg; the overall recoveries for benzimidazoles were >73%, with coefficients of variation below 10%.
建立了一种用于测定动物饲料中五种苯并咪唑（噻苯达唑、阿苯达唑、芬苯达唑、甲苯达唑、奥芬达唑）的高效毛细管电泳方法。分离在一根80.5 cm×75 μm内径的未涂层熔融石英毛细管中进行，通过流体动力学进样（35 mbar×8 s）进样，检测波长为295 nm。药物在50 mmol/L柠檬酸缓冲液（pH 1.9）中，于25℃、30 kV电压下完全分离。线性范围为1 - 80 μg/mL；分析物的检测限小于2 mg/kg；苯并咪唑的总回收率>73%，变异系数低于10%。
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