伪狂犬病病毒在冻干和储存过程中的稳定性：悬浮介质的影响
Stability of pseudorabies virus during freeze-drying and storage: effect of suspending media.
作者信息
Scott E M, Woodside W
出版信息
J Clin Microbiol. 1976 Jul;4(1):1-5. doi: 10.1128/jcm.4.1.1-5.1976.
Abstract
The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamate, either alone or in combination with sucrose and either dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and upon storage. Some possible functions of these additives in preventing injury to the virus during freezing and drying have been suggested.
摘要
研究了悬浮介质对伪狂犬病病毒冻干及后续储存稳定性的影响。测试了多种介质，包括：谷氨酸钠；蔗糖；乳糖；水解乳白蛋白；蛋白胨；蔗糖、右旋糖酐和谷氨酸的组合；以及蔗糖、谷氨酸和磷酸钾的各种组合。含有谷氨酸的悬浮介质，无论是单独使用还是与蔗糖以及右旋糖酐或磷酸盐组合使用，在冻干过程和储存期间都能提供最大程度的保护。已提出这些添加剂在防止病毒在冷冻和干燥过程中受到损伤方面的一些可能作用。
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2
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J Bacteriol. 1950 Apr;59(4):509-22. doi: 10.1128/jb.59.4.509-522.1950.
3
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U S Armed Forces Med J. 1950 Apr;1(4):415-7.
4
5
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J Pharm Pharmacol. 1962 Oct;14:621-40. doi: 10.1111/j.2042-7158.1962.tb11153.x.
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Stud Cercet Inframicrobiol. 1956 Jan-Jun;7(1-2):111-7.
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