由阴道鼠李糖乳杆菌产生的抗菌肽Lactocin 160的安全性研究。
Safety study of an antimicrobial peptide lactocin 160, produced by the vaginal Lactobacillus rhamnosus.
作者信息
Dover Sara E, Aroutcheva Alla A, Faro S, Chikindas Michael L
机构信息
School of Biotechnology, The Royal Institute of Technology, 100 44 Stockholm, Sweden.
出版信息
Infect Dis Obstet Gynecol. 2007;2007:78248. doi: 10.1155/2007/78248.
OBJECTIVE
To evaluate the safety of the antimicrobial peptide, lactocin 160.
METHODS
Lactocin 160, a product of vaginal probiotic Lactobacillus rhamnosus 160 was evaluated for toxicity and irritation. An in vitro human organotypic vaginal-ectocervical tissue model (EpiVaginal) was employed for the safety testing by determining the exposure time to reduce tissue viability to 50% (ET-50). Hemolytic activity of lactocin160 was tested using 8% of human erythrocyte suspension. Susceptibility of lactobacilli to lactocin160 was also studied. Rabbit vaginal irritation (RVI) model was used for an in vivo safety evaluation.
RESULTS
The ET-50 value was 17.5 hours for lactocin 160 (4.9 hours for nonoxynol 9, N9). Hemolytic activity of lactocin 160 was 8.2% (N9 caused total hemolysis). Lactobacilli resisted to high concentrations of peptide preparation. The RVI model revealed slight vaginal irritation. An average irritation index grade was evaluated as "none."
CONCLUSIONS
Lactocin 160 showed minimal irritation and has a good potential for intravaginal application.
目的
评估抗菌肽鼠李糖乳杆菌素160的安全性。
方法
对鼠李糖乳杆菌160产生的鼠李糖乳杆菌素160进行毒性和刺激性评估。采用体外人阴道-宫颈外组织模型（EpiVaginal）通过测定将组织活力降低至50%的暴露时间（ET-50）进行安全性测试。使用8%的人红细胞悬液测试鼠李糖乳杆菌素160的溶血活性。还研究了乳酸杆菌对鼠李糖乳杆菌素160的敏感性。采用兔阴道刺激（RVI）模型进行体内安全性评估。
结果
鼠李糖乳杆菌素160的ET-50值为17.5小时（壬苯醇醚9，N9为4.9小时）。鼠李糖乳杆菌素160的溶血活性为8.2%（N9导致完全溶血）。乳酸杆菌对高浓度的肽制剂具有抗性。RVI模型显示有轻微的阴道刺激。平均刺激指数等级评估为“无”。
结论
鼠李糖乳杆菌素160显示出最小的刺激性，具有良好的阴道内应用潜力。
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