College of Veterinary Medicine, Jilin Agricultural University, 130118, Changchun, Jilin, China.
Department of Animal Sciences, Shaheed Benazir Bhutto University Sheringal Dir Upper-Pakistan, Sheringal, Pakistan.
Microb Biotechnol. 2022 Sep;15(9):2337-2350. doi: 10.1111/1751-7915.14096. Epub 2022 Jul 18.
Pseudomonas sp. strain 166 was isolated from soil samples from Changbai Mountains. A novel bacteriocin PA166 from Pseudomonas sp. 166 was purified using ammonium sulfate, dextran gel chromatography column and Q-Sepharose column chromatography successively. The molecular mass of bacteriocin PA166 was found to be 49.38 kDa by SDS-PAGE and liquid chromatography-mass spectrometry (MS)/MS. Bacteriocin PA166 showed stability at a wide range of pH (2-10), and thermal stability (40, 60, 80 and 100°C). The bacteriocin PA166 antimicrobial activity was slightly inhibited by Ca , K and Mg . The minimum bactericidal concentrations of bacteriocin PA166 against five Pasteurella multocida strains ranged from 2 to 8 μg ml . Bacteriocin PA166 showed low cytotoxicity and a higher treatment index (TI = 82.51). Fluorescence spectroscopy indicated that bacteriocin PA166 destroyed the cell membrane to exert antimicrobial activity. In summary, bacteriocin PA166 had strong antibacterial activity, high TI and low toxicity, and hence could serve as a potential clinical therapeutic drug.
从长白山土壤样本中分离到一株假单胞菌菌株 166。通过硫酸铵沉淀、葡聚糖凝胶色谱柱和 Q 琼脂糖柱色谱法，从假单胞菌 166 中纯化出一种新型细菌素 PA166。SDS-PAGE 和液相色谱-质谱/质谱（MS/MS）结果表明，细菌素 PA166 的分子量为 49.38 kDa。PA166 对 pH 值在 2-10 之间和在 40、60、80 和 100°C 下都有很好的稳定性。该细菌素对 Ca 、K 和 Mg 的抑菌活性有轻微抑制作用。PA166 对 5 株多杀性巴氏杆菌的最小杀菌浓度范围为 2-8 μg/ml。PA166 对细胞的最低毒性和治疗指数（TI）分别为 82.51。荧光光谱表明，PA166 破坏细胞膜发挥抗菌活性。综上所述，细菌素 PA166 具有较强的抗菌活性、高 TI 和低毒性，因此可作为一种有潜在临床应用价值的治疗药物。
