Pascual Liliana M, Daniele María B, Ruiz Francisco, Giordano Walter, Pájaro Cristina, Barberis Lucila
Departamento de Microbiología e Inmunología, Universidad Nacional de Rio Cuarto, Cordoba, Argentina.
J Gen Appl Microbiol. 2008 Jun;54(3):141-8. doi: 10.2323/jgam.54.141.
The vagina has been increasingly viewed as an "ecosystem" whose normal microflora help protect it from invading pathogens, including those that cause urinary tract infections and sexually transmitted diseases. We tested new strains of lactobacilli as potential probiotics for maintenance of urogenital tract health, as well as prevention and therapy of urogenital infections. A strain of lactobacilli isolated from the vagina of nonpregnant, healthy, premenopausal women was identified as Lactobacillus rhamnosus L60 by 16S rDNA sequence homology. L60 was evaluated for antimicrobial activity, in vitro antibiotic resistance, autoaggregation, surface hydrophobicity, co-aggregation with other bacterial species, hydrogen peroxide (H(2)O(2)) production, and bacterial adherence. It displayed a wide spectrum of antimicrobial activity against urogenital pathogens, and resistance to antibiotics commonly prescribed for infections caused by these pathogens. L60 produced H(2)O(2), adhered to vaginal epithelial cells, co-aggregated with Escherichia coli and Candida albicans, and displayed self-aggregation. In view of these characteristics, L60 is considered a potential probiotic, and will be further evaluated for preventive and therapeutic application locally in the vaginal tract.
阴道越来越被视为一个“生态系统”，其正常微生物群有助于保护它免受入侵病原体的侵害，这些病原体包括那些引起尿路感染和性传播疾病的病原体。我们测试了新型乳酸杆菌菌株作为维持泌尿生殖道健康以及预防和治疗泌尿生殖道感染的潜在益生菌。通过16S rDNA序列同源性，从非妊娠、健康、绝经前妇女阴道分离出的一株乳酸杆菌被鉴定为鼠李糖乳杆菌L60。对L60进行了抗菌活性、体外抗生素耐药性、自聚集、表面疏水性、与其他细菌种类的共聚集、过氧化氢（H₂O₂）产生以及细菌黏附等方面的评估。它对泌尿生殖道病原体表现出广泛的抗菌活性，并且对这些病原体引起的感染常用的抗生素具有耐药性。L60产生H₂O₂，黏附于阴道上皮细胞，与大肠杆菌和白色念珠菌共聚集，并表现出自聚集。鉴于这些特性，L60被认为是一种潜在的益生菌，并将进一步评估其在阴道局部预防和治疗应用中的效果。
