SSEA-4在小鼠植入前胚胎卵裂球之间界面的优先定位。
Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.
作者信息
Sato Ban, Katagiri Yohko U, Miyado Kenji, Akutsu Hidenori, Miyagawa Yoshitaka, Horiuchi Yasuomi, Nakajima Hideki, Okita Hajime, Umezawa Akihiro, Hata Jun-Ichi, Fujimoto Junichiro, Toshimori Kiyotaka, Kiyokawa Nobutaka
机构信息
Department of Developmental Biology, National Research Institute for Child Health and Development, 2-1 0-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
出版信息
Biochem Biophys Res Commun. 2007 Dec 28;364(4):838-43. doi: 10.1016/j.bbrc.2007.10.093. Epub 2007 Oct 26.
The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.
针对胚胎癌细胞系NCR - G3产生的单克隆抗体6E2已被证明也能与人生殖细胞发生反应。薄层色谱（TLC）免疫染色显示，6E2与唾液酸化球蛋白五糖神经酰胺（sialylGb5）特异性反应，后者携带阶段特异性胚胎抗原-4（SSEA - 4）的表位，SSEA - 4是胚胎发育过程中一种重要的细胞表面标志物。对未固定的小鼠植入前胚胎进行免疫染色显示，6E2的结合导致唾液酸化球蛋白五糖神经酰胺在卵裂球之间的界面处聚集并随之积累。这些结果表明，SSEA - 4在细胞表面活跃移动，并在6E2结合后容易在卵裂球之间积累。
Zotero 插件