Costa Monya M, Figueiredo A Cristina, Barroso José G, Pedro Luis G, Deans Stanley G, Scheffer Johannes J C
Universidade de Lisboa, Faculdade de Ciências de Lisboa, Dep. de Biologia Vegetal, Centro de Biotecnologia Vegetal, 1749-016, Lisbon, Portugal.
Biotechnol Lett. 2008 Jul;30(7):1265-70. doi: 10.1007/s10529-008-9664-y. Epub 2008 Feb 14.
Six-year-old Levisticum officinale (lovage) hairy root cultures were used to study the effect of eight different NH(4) (+):NO(3) (-) ratios on their growth and volatile components. All cultures were kept at 24 degrees C on orbital shakers at 80 rpm, in darkness or in a 16 h light/8 h dark photoperiod. Growth was evaluated by dry and fresh weight determination. The volatiles were isolated by distillation-extraction and analysed by GC and GC-MS. Greater growth was attained in darkness with 10:90 (control, SH medium), 50:50 and 25:75 NH(4) (+):NO(3) (-) ratios, and also with SH control medium under the photoperiod condition, with a 10, 14, 12.5 and 12.5 fold increase of biomass in terms of dry weight, respectively, at the end of 42 days of growth. UPGMA cluster analysis of the mixtures of volatiles isolated from the hairy roots grown with different NH(4) (+):NO(3) (-) ratios confirmed their chemical variability. Although no particular grouping was detected in relation to the NH(4) (+):NO(3) (-) ratios or light conditions studied, most of the mixtures of volatiles isolated from the hairy roots were either dominated by n-octanal, (Z)-falcarinol or both components in about the same relative amounts.
利用6岁的药用欧芹（lovage）毛状根培养物研究8种不同的NH₄⁺:NO₃⁻比例对其生长和挥发性成分的影响。所有培养物在24℃下于轨道摇床上以80转/分钟振荡培养，培养条件为黑暗或16小时光照/8小时黑暗光周期。通过测定干重和鲜重评估生长情况。通过蒸馏萃取分离挥发性成分，并采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）进行分析。在黑暗条件下，NH₄⁺:NO₃⁻比例为10:90（对照，SH培养基）、50:50和25:75时生长较好，在光周期条件下SH对照培养基中生长也较好，在生长42天结束时，干重生物量分别增加了10倍、14倍、12.5倍和12.5倍。对从不同NH₄⁺:NO₃⁻比例培养的毛状根中分离出的挥发性成分混合物进行的UPGMA聚类分析证实了它们的化学变异性。尽管在所研究的NH₄⁺:NO₃⁻比例或光照条件方面未检测到特定的分组，但从毛状根中分离出的大多数挥发性成分混合物要么以正辛醛、(Z)-法卡林醇为主，要么这两种成分的相对含量大致相同。
