Faria Jorge M S, Nunes Inês S, Figueiredo A Cristina, Pedro Luis G, Trindade Helena, Barroso José G
Faculdade de Ciências de Lisboa, Departamento de Biologia Vegetal, Instituto de Biotecnologia e Bioengenharia, Centro de Biotecnologia Vegetal, Universidade de Lisboa, C2, Campo Grande, 1749-016 Lisbon, Portugal.
Biotechnol Lett. 2009 Jun;31(6):897-903. doi: 10.1007/s10529-009-9934-3. Epub 2009 Feb 11.
Two oxygen-containing monoterpene substrates, menthol or geraniol (25 mg l(-1)), were added to Anethum graveolens hairy root cultures to evaluate the influence of the biotransformation capacity on growth and production of volatile compounds. Growth was assessed by the dissimilation method and by fresh and dry weight measurement. The volatiles were analyzed by GC and GC-MS. The total constitutive volatile component was composed, in more than 50%, by falcarinol (17-52%), apiole (11-24%), palmitic acid (7-16%), linoleic acid (4-9%), myristicin (4-8%) and n-octanal (2-5%). Substrate addition had no negative influence on growth. The relative amount of menthol quickly decreased 48 h after addition, and the biotransformation product menthyl acetate was concomitantly formed. Likewise, the added geraniol quickly decreased over 48 h alongside with the production of the biotransformation products. The added geraniol was biotransformed in 10 new products, the alcohols linalool, alpha-terpineol and citronellol, the aldehydes neral and geranial, the esters citronellyl, neryl and geranyl acetates and linalool and nerol oxides.
将两种含氧单萜底物，薄荷醇或香叶醇（25 mg l(-1)）添加到莳萝毛状根培养物中，以评估生物转化能力对挥发性化合物生长和产生的影响。通过异化方法以及鲜重和干重测量来评估生长情况。通过气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）分析挥发性化合物。总的组成挥发性成分中，超过50%由镰叶芹醇（17 - 52%）、芹菜脑（11 - 24%）、棕榈酸（7 - 16%）、亚油酸（4 - 9%）、肉豆蔻醚（4 - 8%）和正辛醛（2 - 5%）组成。添加底物对生长没有负面影响。添加薄荷醇后48小时，其相对含量迅速下降，同时生物转化产物乙酸薄荷酯形成。同样，添加的香叶醇在48小时内迅速减少，同时产生生物转化产物。添加的香叶醇被生物转化为10种新产物，即醇类芳樟醇、α - 松油醇和香茅醇，醛类橙花醛和香叶醛，酯类香茅基、橙花基和香叶基乙酸酯以及芳樟醇和橙花叔醇氧化物。
