Demir Elif, Taysi Seyithan, Al Behcet, Demir Tuncer, Okumus Seydi, Saygili Oguzhan, Saricicek Edibe, Dirier Ahmet, Akan Muslum, Tarakcioglu Mehmet, Bagci Cahit
Department of Medical Biochemistry, Medical School, Gaziantep University, Gaziantep, Turkey.
Department of Emergency Medicine, Medical School, Gaziantep University, Gaziantep, Turkey.
Wien Klin Wochenschr. 2016 Dec;128(Suppl 8):587-595. doi: 10.1007/s00508-015-0736-4. Epub 2015 Apr 10.
The aim of this study was to investigate the antioxidant and radioprotective effects of propolis, caffeic acid phenethyl ester (CAPE), Nigella sativa oil (NSO), and thymoquinone (TQ) against ionizing radiation-induced cataracts in lens after total cranium irradiation of rats with single dose of 5-Gy cobalt-60 gamma rays.
A total of 74 Sprague-Dawley rats were divided into 8 groups to test the radioprotective effectiveness of Nigella sativa oil, thymoquine, propolis, or caffeic acid phenethyl ester administered by either orogastric tube or intraperitoneal injection. Appropriate control groups were also studied.
Chylack's cataract classification was used in the study. At the end of the tenth day, cataracts developed in 80 % of the rats in the radiotherapy group. After irradiation, cataract rate dropped to 20 % in NSO, 30 % in propolis, 40 % in CAPE, and 50 % in TQ groups and was limited to grade 1 and grade 2. Cataract formation was observed the least in NSO group and the most in TQ group. In the irradiated (IR) group, superoxide dismutase activity was lower, while glutathione peroxidase and xanthine oxidase activities and malondialdehyde level were higher compared with the other groups. Total superoxide scavenger activity and nonenzymatic superoxide scavenger activity were not statistically significant in IR group compared with the other groups.
The findings obtained in the study might suggest that propolis, CAPE, NSO, and TQ could prevent cataractogenesis in ionizing radiation-induced cataracts in the lenses of rats, wherein propolis and NSO were found to be more potent.
本研究旨在探讨蜂胶、咖啡酸苯乙酯（CAPE）、黑种草籽油（NSO）和百里醌（TQ）对单次剂量5-Gy钴-60γ射线全颅照射大鼠晶状体电离辐射诱导性白内障的抗氧化和辐射防护作用。
总共74只斯普拉格-道利大鼠被分为8组，以测试经口胃管或腹腔注射给予黑种草籽油、百里醌、蜂胶或咖啡酸苯乙酯的辐射防护效果。还研究了适当的对照组。
本研究采用齐拉克白内障分级。在第十天结束时，放疗组80%的大鼠出现白内障。照射后，NSO组白内障发生率降至20%，蜂胶组为30%，CAPE组为40%，TQ组为50%，且仅限于1级和2级。NSO组白内障形成最少，TQ组最多。与其他组相比，照射（IR）组超氧化物歧化酶活性较低，而谷胱甘肽过氧化物酶、黄嘌呤氧化酶活性和丙二醛水平较高。与其他组相比，IR组的总超氧化物清除剂活性和非酶促超氧化物清除剂活性无统计学意义。
本研究结果可能表明，蜂胶、CAPE、NSO和TQ可预防大鼠晶状体电离辐射诱导性白内障的发生，其中发现蜂胶和NSO的效果更强。
