Alhaj-Qasem Dina M, Al-Hatamleh Mohammad A I, Irekeola Ahmad Adebayo, Khalid Muhammad Fazli, Mohamud Rohimah, Ismail Aziah, Mustafa Fatin Hamimi
Precision Medical Laboratory, Zahran, Amman 11183, Jordan.
Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia.
Diagnostics (Basel). 2020 Jun 28;10(7):438. doi: 10.3390/diagnostics10070438.
Paratyphoid fever is caused by the bacterium serovar Paratyphi (A, B and C), and contributes significantly to global disease burden. One of the major challenges in the diagnosis of paratyphoid fever is the lack of a proper gold standard. Given the absence of a licensed vaccine against Paratyphi, this diagnostic gap leads to inappropriate antibiotics use, thus, enhancing antimicrobial resistance. In addition, the symptoms of paratyphoid overlap with other infections, including the closely related typhoid fever. Since the development and utilization of a standard, sensitive, and accurate diagnostic method is essential in controlling any disease, this review discusses a new promising approach to aid the diagnosis of paratyphoid fever. This advocated approach is based on the use of surface plasmon resonance (SPR) biosensor and DNA probes to detect specific nucleic acid sequences of Paratyphi. We believe that this SPR-based genoassay can be a potent alternative to the current conventional diagnostic methods, and could become a rapid diagnostic tool for paratyphoid fever.
副伤寒是由副伤寒杆菌血清型（A、B和C）引起的，对全球疾病负担有重大影响。副伤寒诊断的主要挑战之一是缺乏合适的金标准。由于缺乏针对副伤寒的许可疫苗，这种诊断差距导致抗生素使用不当，从而增强了抗菌耐药性。此外，副伤寒的症状与其他感染重叠，包括密切相关的伤寒。由于开发和使用标准、灵敏且准确的诊断方法对于控制任何疾病至关重要，本综述讨论了一种有助于副伤寒诊断的新的有前景的方法。这种提倡的方法基于使用表面等离子体共振（SPR）生物传感器和DNA探针来检测副伤寒杆菌的特定核酸序列。我们相信这种基于SPR的基因检测法可以成为当前传统诊断方法的有力替代方法，并可能成为副伤寒的快速诊断工具。
