人类胚胎干细胞系之间分化倾向存在显著差异。

Marked differences in differentiation propensity among human embryonic stem cell lines.

作者信息

Osafune Kenji, Caron Leslie, Borowiak Malgorzata, Martinez Rita J, Fitz-Gerald Claire S, Sato Yasunori, Cowan Chad A, Chien Kenneth R, Melton Douglas A

机构信息

Harvard Stem Cell Institute, 42 Church Street, Cambridge, Massachusetts 02138, USA.

出版信息

Nat Biotechnol. 2008 Mar;26(3):313-5. doi: 10.1038/nbt1383. Epub 2008 Feb 17.

DOI:10.1038/nbt1383

PMID:18278034

Abstract

The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

摘要

比较了17个人类胚胎干细胞（hES）系的分化潜能。一些细胞系表现出明显的分化为特定谱系的倾向，谱系特异性基因表达常常有超过100倍的差异。例如，HUES 8最适合胰腺分化，而HUES 3则适合生成心肌细胞。hES细胞系之间这些显著的发育潜能差异表明筛选和建立用于谱系特异性分化的细胞系的重要性。