Saganová Kamila, Orendácová Judita, Cízková Dása, Vanický Ivo
Institute of Neurobiology, Center of Excellence, Slovak Academy of Sciences, Soltésovej 6, 040 01 Kosice, Slovak Republic.
Neurosci Lett. 2008 Mar 15;433(3):246-9. doi: 10.1016/j.neulet.2008.01.041. Epub 2008 Jan 20.
Minocycline (MC), a second-generation tetracycline and anti-inflammatory agent reportedly provides neuroprotection following CNS injury. The objective of this study was to examine the neuroprotective effects of short and long-term MC treatment using balloon-compression spinal cord injury (SCI) in the rat. Rats subjected to SCI were treated with MC for 1 day (1DMC group; total dose 180 mg/kg) or 5 days (5DMC group; total dose 450 mg/kg) or placebo. The effects of MC treatment on locomotor recovery (BBB scale) and spinal cord white and gray matter sparing were evaluated for up to 28 days. Morphometric analysis showed that while MC treatment spared spinal cord white and gray matter rostral to the lesion epicenter in both, 1DMC and 5DMC groups, sparing of white and gray matter areas was not observed caudal to the traumatic lesion. In addition, MC treatment had no effect on final locomotor recovery. Limited improvement of spinal cord post-compression consequences raises questions about the neuroprotection efficiency of MC treatment following compression SCI in the rat.
米诺环素（MC）是一种第二代四环素类抗炎药，据报道，它在中枢神经系统损伤后具有神经保护作用。本研究的目的是使用大鼠球囊压迫性脊髓损伤（SCI）模型，研究短期和长期米诺环素治疗的神经保护作用。将遭受脊髓损伤的大鼠分为三组，分别给予米诺环素治疗1天（1DMC组；总剂量180mg/kg）或5天（5DMC组；总剂量450mg/kg）或安慰剂。评估米诺环素治疗对运动功能恢复（BBB评分）以及脊髓白质和灰质保留情况的影响，观察期长达28天。形态学分析表明，1DMC组和5DMC组中，米诺环素治疗均能使损伤震中前方的脊髓白质和灰质得以保留，但在创伤性损伤后方未观察到白质和灰质区域的保留。此外，米诺环素治疗对最终的运动功能恢复没有影响。脊髓压迫后后果的改善有限，这引发了人们对大鼠压迫性脊髓损伤后米诺环素治疗神经保护效率的质疑。
