Izal-Azcárate Amaya, Belzunegui Silvia, San Sebastián Waldy, Garrido-Gil Pablo, Vázquez-Claverie Marianne, López Berta, Marcilla Irene, Luquin M A Rosario
Department of Neuroscience, Centre for Applied Medical Research-University of Navarra, Avda. Pío XII 55, 31008 Pamplona, Navarra, Spain.
Respir Physiol Neurobiol. 2008 Mar 20;161(1):95-9. doi: 10.1016/j.resp.2007.12.008. Epub 2008 Jan 8.
We studied the histochemical phenotype of carotid body (CB) cells in the adult rat. In addition to tyrosine hydroxylase (TH), type I cells expressed numerous growth factors such as glial cell line-derived neurotrophic factor (GDNF), basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha), as well as the receptors p75, Ret, epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor-alpha (PDGFR-alpha). Type II cells expressed the glial fibrillary acid protein (GFAP), vimentin, the trophic factor bFGF and receptors p75, EGFR and PDGFR-alpha. Both types I and II cells exhibited a positive immunoreaction to markers of neural progenitor cells such as the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) and nestin, respectively, suggesting that CB contain some immature cells even at the adult stage. The possibility that these cells can be expanded and differentiated into mature neurons should be explored.
我们研究了成年大鼠颈动脉体（CB）细胞的组织化学表型。除酪氨酸羟化酶（TH）外，I型细胞还表达多种生长因子，如胶质细胞源性神经营养因子（GDNF）、碱性成纤维细胞生长因子（bFGF）、脑源性神经营养因子（BDNF）、睫状神经营养因子（CNTF）、胰岛素样生长因子-I（IGF-I）、表皮生长因子（EGF）和转化生长因子-α（TGF-α），以及受体p75、Ret、表皮生长因子受体（EGFR）和血小板源性生长因子受体-α（PDGFR-α）。II型细胞表达胶质纤维酸性蛋白（GFAP）、波形蛋白、营养因子bFGF以及受体p75、EGFR和PDGFR-α。I型和II型细胞分别对神经祖细胞标志物如多唾液酸化神经细胞黏附分子（PSA-NCAM）和巢蛋白呈现阳性免疫反应，这表明即使在成年阶段，CB中也含有一些未成熟细胞。应该探索这些细胞能否扩增并分化为成熟神经元的可能性。
