Braithwaite A W, Nelson C C, Bellett A J
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra ACT.
New Biol. 1991 Jan;3(1):18-26.
Products encoded in the E1a oncogene of adenoviruses are required to activate transcription of all viral early genes and some cellular genes. A current interpretation of experimental data supports the hypothesis that this "trans-activation" is mediated solely by a block of amino acids known as conserved domain 3, which is unique to the largest E1a protein, while the remaining E1a protein sequences contain discrete domains required for functions other than trans-activation. However, there is also considerable evidence inconsistent with this simple model of E1a structure and function. Both of the major E1a proteins appear to participate in trans-activation by three different types of interaction with cellular transcription factors and other regulatory proteins. In this review we attempt to rationalize the experimental data and provide a more integrated view of E1a structure and function.
腺病毒E1a癌基因编码的产物是激活所有病毒早期基因和一些细胞基因转录所必需的。目前对实验数据的一种解释支持这样一种假说，即这种“反式激活”仅由一段称为保守结构域3的氨基酸序列介导，该序列是最大的E1a蛋白所特有的，而其余的E1a蛋白序列包含反式激活以外功能所需的离散结构域。然而，也有相当多的证据与这种简单的E1a结构和功能模型不一致。两种主要的E1a蛋白似乎都通过与细胞转录因子和其他调节蛋白的三种不同类型的相互作用参与反式激活。在这篇综述中，我们试图使实验数据合理化，并提供一个关于E1a结构和功能的更综合的观点。
