Nico Beatrice, Benagiano Vincenzo, Mangieri Domenica, Maruotti Nicola, Vacca Angelo, Ribatti Domenico
Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.
Histol Histopathol. 2008 May;23(5):601-7. doi: 10.14670/HH-23.601.
Microvascular density (MVD) counting protocols have become the morphological gold standard to assess the neovasculature in human tumors. This method requires the use of specific markers to vascular endothelium and of immunohistochemical procedures to visualize microvessels. MVD determined in primary tumors is significantly associated with metastasis and prognosis in several tumors and is most predictive in those tumors that induce significant angiogenesis, namely carcinomas of breast and prostate, and haematological malignancies. There is such a wide range of antibodies and suppliers, antigen retrieval methods, designation of high and low vessel count groups, patient study groups and data interpretation, that it is exceedingly difficult to compare results.
微血管密度（MVD）计数方案已成为评估人类肿瘤新生血管的形态学金标准。该方法需要使用针对血管内皮的特定标志物以及免疫组织化学程序来使微血管可视化。在原发性肿瘤中测定的MVD与多种肿瘤的转移和预后显著相关，并且在那些诱导显著血管生成的肿瘤中预测性最强，即乳腺癌和前列腺癌以及血液系统恶性肿瘤。由于抗体和供应商种类繁多、抗原修复方法各异、高低血管计数组的指定不同、患者研究组不同以及数据解释不同，因此结果极难比较。
