使用光声显微镜和漫射光学光谱对肿瘤异种移植的血管生成和氧合进行体内监测。
In vivo monitoring of vascularization and oxygenation of tumor xenografts using optoacoustic microscopy and diffuse optical spectroscopy.
作者信息
Akhmedzhanova K G, Kurnikov A A, Khochenkov D A, Khochenkova Yu A, Glyavina A M, Kazakov V V, Yudintsev A V, Maslennikova A V, Turchin I V, Subochev P V, Orlova A G
机构信息
Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia.
Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia.
出版信息
Biomed Opt Express. 2022 Oct 11;13(11):5695-5708. doi: 10.1364/BOE.469380. eCollection 2022 Nov 1.
The research is devoted to comparison of the blood vessel structure and the oxygen state of three xenografts: SN-12C, HCT-116 and Colo320. Differences in the vessel formation and the level of oxygenation are revealed by optoacoustic (OA) microscopy and diffuse optical spectroscopy (DOS) respectively. The Colo320 tumor is characterized by the highest values of vessel size and fraction. DOS showed increased content of deoxyhemoglobin that led to reduction of saturation level for Colo320 as compared to other tumors. Immunohistochemical (IHC) analysis for CD31 demonstrates the higher number of vessels in Colo320. The IHC for hypoxia was consistent with DOS results and revealed higher values of the relative hypoxic fraction in Colo320.
该研究致力于比较三种异种移植瘤（SN - 12C、HCT - 116和Colo320）的血管结构和氧状态。分别通过光声（OA）显微镜和漫射光学光谱（DOS）揭示了血管生成和氧合水平的差异。Colo320肿瘤的特征是血管大小和比例值最高。DOS显示脱氧血红蛋白含量增加，导致Colo320与其他肿瘤相比饱和度水平降低。对CD31的免疫组织化学（IHC）分析表明Colo320中的血管数量更多。缺氧的IHC结果与DOS结果一致，显示Colo320中相对缺氧分数的值更高。
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