Yang Xiao-Feng, Han Xue-Bing, Rong Dong-Wen, Li De-Biao
Department of Urology, the First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Zhonghua Nan Ke Xue. 2007 Dec;13(12):1072-4.
To investigate the impact of hCG on the epidermal growth factor (EGF) in the phallic tissues of hypospadiac mice.
The Kunming mouse model of congenital hypospadias was established. As controls, 10 three-week-old normal male mice and 10 3-week-old congenital hypospadiac mice were injected with isometric sodium chloride intraperitoneally. Another 40 mice with hypospadias were divided into 4 groups and injected intraperitoneally with 50 IU, 100 IU, 150 IU and 200 IU of hCG respectively per day for 7 days. Then the concentration of ECG in the phallus and in the serum were assayed by ELISA.
The EGF level in the phallus of the hypospadiac mice was much lower than that of the controls statistically (P < 0. 05). After the injection of exogenous hCG, the concentration of ECG increased with the dose of hCG, 50 IU (59.57 +/- 22.16) pg/ml, 100 IU (57.97 +/- 9.59) pg/ml, 150 IU (73.02 +/- 31.35) pg/ml and 200 IU (92.45 +/- 35.74) pg/ml. The concentration of ECG showed no significant difference between the control group and the 150 IU and 2000 IU groups (P > 0.05).
Decreased concentration of EGF in the mouse phallus may be associated with the pathogeny of hypospadias. A proper dose of exogenous hCG may increase the concentration of EGF in the mouse phallus.
探讨人绒毛膜促性腺激素（hCG）对尿道下裂小鼠阴茎组织中表皮生长因子（EGF）的影响。
建立昆明小鼠先天性尿道下裂模型。作为对照，将10只3周龄正常雄性小鼠和10只3周龄先天性尿道下裂小鼠腹腔注射等体积的氯化钠。另外40只尿道下裂小鼠分为4组，分别每天腹腔注射50 IU、100 IU、150 IU和200 IU的hCG，共7天。然后采用酶联免疫吸附测定法（ELISA）检测阴茎和血清中表皮生长因子（ECG）的浓度。
尿道下裂小鼠阴茎中的EGF水平在统计学上显著低于对照组（P < 0.05）。注射外源性hCG后，ECG浓度随hCG剂量增加而升高，50 IU组为（59.57 ± 22.16）pg/ml，100 IU组为（57.97 ± 9.59）pg/ml，150 IU组为（73.02 ± 31.35）pg/ml，200 IU组为（92.45 ± 35.74）pg/ml。对照组与150 IU组和2000 IU组之间的ECG浓度无显著差异（P > 0.05）。
小鼠阴茎中EGF浓度降低可能与尿道下裂的发病机制有关。适当剂量的外源性hCG可能会增加小鼠阴茎中EGF的浓度。
