枯草芽孢杆菌flaA基因座的遗传分析。
Genetic analysis of the flaA locus of Bacillus subtilis.
作者信息
Hauser P M, Crabb W D, Fiora M G, Scoffone F, Galizzi A
机构信息
Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Università degli Studi di Pavia, Italy.
出版信息
J Bacteriol. 1991 Jun;173(11):3580-3. doi: 10.1128/jb.173.11.3580-3583.1991.
Abstract
We isolated two clones of recombinant lambda bacteriophage with overlapping inserts of Bacillus subtilis chromosomal DNA corresponding to part of the flaA locus. The flaA4 and flaA15 mutations were localized on the physical map by marker rescue experiments. The flaA locus and the flaB (sigD) gene were mapped in transduction crosses, and the order glnA polC flaB flaA was determined. FlaB was linked to polC in transformation crosses.
摘要
我们分离出了两个重组λ噬菌体克隆，其插入的枯草芽孢杆菌染色体DNA片段相互重叠，对应于flaA基因座的一部分。通过标记拯救实验将flaA4和flaA15突变定位到物理图谱上。在转导杂交中对flaA基因座和flaB（sigD）基因进行了定位，并确定了基因顺序为glnA polC flaB flaA。在转化杂交中，FlaB与polC相连。
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