枯草芽孢杆菌核黄素操纵子调控突变的遗传图谱分析
Genetic mapping of regulatory mutations of Bacillus subtilis riboflavin operon.
作者信息
Kreneva R A, Perumov D A
机构信息
Department of Molecular and Radiation Biophysics, Leningrad Nuclear Physic Institute, Academy of Sciences, USSR.
出版信息
Mol Gen Genet. 1990 Jul;222(2-3):467-9. doi: 10.1007/BF00633858.
Seven mutations leading to riboflavin overproduction in Bacillus subtilis were found to be linked to the marker dnaF133 (145 degrees on the B. subtilis genetic map) by transformation. Cotransfer indexes (42.5%-61.7%) suggest that the ribC mutations are alleles of the same locus. Results of transduction and transformation crosses suggest the following order of markers:pyrD26-ts-6-dnaF133-ribC-recA1.
通过转化发现，在枯草芽孢杆菌中导致核黄素过量产生的7个突变与标记dnaF133（在枯草芽孢杆菌遗传图谱上位于145度处）连锁。共转移指数（42.5%-61.7%）表明ribC突变是同一基因座的等位基因。转导和转化杂交结果表明标记的顺序如下：pyrD26-ts-6-dnaF133-ribC-recA1。
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