Montag M, Schimming T, Köster M, Zhou C, Dorn C, Rösing B, van der Ven H, Ven der Ven K
Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn, Sigmund-Freud-Str. 25, D-53127 Bonn, Germany.
Reprod Biomed Online. 2008 Feb;16(2):239-44. doi: 10.1016/s1472-6483(10)60580-9.
A retrospective study recently showed that oocytes presenting with a high birefringence of the inner zona layer were more often associated with conception cycles. To further investigate these findings, a prospective study was conducted between September 2005 and September 2006 including intracytoplasmic sperm injection (ICSI) cycles presenting with at least two embryos for transfer. Using a polarization imaging system, oocytes were classified prior to ICSI treatment as having either a high zona birefringence (HZB) or a low zona birefringence (LZB) of the zona pellucida. Using zona birefringence as the only selection criterion, two fertilized oocytes, preferably derived from HZB oocytes, were selected for further culture and transfer. The required criteria were met by 135 ICSI cycles (124 patients; 34.9 +/- 4.1 years of age). Embryos for transfer were used in 20 cycles derived from HZB/HZB oocytes, in 50 cycles from HZB/LZB oocytes and in 65 from LZB/LZB oocytes. The corresponding implantation (P < 0.025), pregnancy (P < 0.005) and live birth (P < 0.025) rates were significantly different between HZB/HZB and HZB/LZB versus LZB/LZB group. Embryo development was superior in embryos derived from HZB oocytes. This study concludes that oocyte zona birefringence is a good selection criterion and a good predictive criterion for embryo implantation potential.
最近一项回顾性研究表明，透明带内层双折射高的卵母细胞更常与受孕周期相关。为进一步研究这些发现，于2005年9月至2006年9月进行了一项前瞻性研究，纳入至少有两个胚胎可供移植的卵胞浆内单精子注射（ICSI）周期。使用偏振成像系统，在ICSI治疗前将卵母细胞分类为透明带双折射高（HZB）或透明带双折射低（LZB）。以透明带双折射作为唯一选择标准，选择两个受精卵，最好来自HZB卵母细胞，进行进一步培养和移植。135个ICSI周期（124例患者；年龄34.9±4.1岁）符合所需标准。来自HZB/HZB卵母细胞的20个周期、HZB/LZB卵母细胞的50个周期和LZB/LZB卵母细胞的65个周期使用了可供移植的胚胎。HZB/HZB和HZB/LZB组与LZB/LZB组之间相应的着床率（P<0.025）、妊娠率（P<0.005）和活产率（P<0.025）有显著差异。来自HZB卵母细胞的胚胎发育更好。本研究得出结论，卵母细胞透明带双折射是胚胎着床潜能的良好选择标准和良好预测标准。
