Marsella Tiziana, Sena Paola, Xella Susanna, La Marca Antonio, Giulini Simone, De Pol Anto, Volpe Annibale, Marzona Laura
Mother-Infant Department, Institute of Obstetrics and Gynaecology, University of Modena and Reggio Emilia, Largo del Pozzo 71, 41100 Modena, Italy.
Reprod Biomed Online. 2008 Feb;16(2):257-67. doi: 10.1016/s1472-6483(10)60583-4.
Recent improvements in techniques in clinical assisted reproduction have led to an increased interest in the cryopreservation of human ovarian tissue as a way of preserving fertility and ovarian steroidogenic activity in young cancer patients. Acceptable follicular survival in frozen-thawed human ovarian tissue has generally been reported. Since a 0.3 mol/l sucrose concentration in cryopreservation solutions evidently increases human oocyte survival after cryopreservation, the aim of this study was to observe the effect of sucrose concentrations of 0.2 mol/l and 0.3 mol/l on human ovarian tissue survival after thawing. Ovarian cortical slices from 10 patients, 22-36 years of age, were cryopreserved slowly using 0.2 mol/l or 0.3 mol/l sucrose with 1,2-propanediol (1.5 mol/l) as the cryoprotectants. Light and electron microscopy were used for the histological analyses. Results showed that both treatments produced an increase in damaged cells; however, the use of 0.3 mol/l sucrose showed a smaller percentage of damaged germ cells than 0.2 mol/l sucrose, and therefore was less detrimental to the thawed ovarian tissue. However as the damage occurred principally in the stroma and follicular cells rather than in the oocytes, the suitability of these cryopreservation protocols must be further evaluated prior to considering the use of stored ovarian cortex for autografting after thawing.
临床辅助生殖技术的最新进展引发了人们对冷冻保存人类卵巢组织的更大兴趣，这是一种在年轻癌症患者中保存生育能力和卵巢类固醇生成活性的方法。一般报道称，冻融后的人类卵巢组织中有可接受的卵泡存活率。由于冷冻保存溶液中0.3mol/L的蔗糖浓度明显提高了冷冻保存后人卵母细胞的存活率，本研究的目的是观察0.2mol/L和0.3mol/L的蔗糖浓度对解冻后人类卵巢组织存活的影响。使用0.2mol/L或0.3mol/L的蔗糖与1,2 - 丙二醇（1.5mol/L）作为冷冻保护剂，对10名年龄在22 - 36岁患者的卵巢皮质切片进行慢速冷冻保存。采用光镜和电镜进行组织学分析。结果显示，两种处理均导致受损细胞增加；然而，使用0.3mol/L蔗糖时受损生殖细胞的比例低于0.2mol/L蔗糖，因此对解冻后的卵巢组织损害较小。然而，由于损伤主要发生在基质和卵泡细胞而非卵母细胞中，在考虑将解冻后的储存卵巢皮质用于自体移植之前，必须进一步评估这些冷冻保存方案的适用性。
