Human Reproductive Medicine Unit, S. Orsola-Malpighi Hospital, University of Bologna, via Massarenti 13, 40138 Bologna, Italy.
Reprod Biomed Online. 2010 Dec;21(6):819-28. doi: 10.1016/j.rbmo.2010.07.008. Epub 2010 Aug 1.
Chemotherapy and/or radiotherapy protocols have improved the long-term survival of cancer patients. Frequent consequences of antiblastic treatments, used to eradicate malignancies, are the partial loss of ovarian function, which in children and young women can result in permanent sterility. Ovarian tissue cryopreservation implemented before the beginning of treatment may potentially restore fertility. However, the physical effects of cryopreservation can damage oocyte survival and decrease follicular cell integrity and stromal preservation. The aim of this study was to examine the effects of different concentrations of 1,2-propanediol (PROH) and sucrose as cryoprotectants and human serum as protein support. Particular concentrations tested were 1.26, 1.5 and 1.08 mol/l PROH, 0.175, 0.2, 0.224 and 0.3 mol/l of sucrose and 20%, 30% and 40% human serum in the freezing solutions and normal or raised sucrose concentrations in the dilution solutions. Ovarian cortical slices from 13 patients, aged 5-38 years, were cryopreserved using slow freezing-rapid thawing. Tests were conducted using light and transmission electron microscopy. Cryo-damage occurred predominantly in the stromal and follicular cells. The best preservation of morphological characteristics was obtained using the freeze-thaw protocol in which concentrations of cryoprotectants were among the lowest (1.26 mol/l PROH+0.175 mol/l sucrose) with 30% human serum.
化疗和/或放疗方案提高了癌症患者的长期生存率。为了消灭恶性肿瘤而使用的抗肿瘤治疗常常导致卵巢功能部分丧失，这在儿童和年轻女性中可能导致永久性不育。在开始治疗前进行卵巢组织冷冻保存可能有潜力恢复生育能力。然而，冷冻保存的物理效应会损害卵母细胞的存活，并降低卵泡细胞的完整性和基质的保存。本研究旨在研究不同浓度的 1,2-丙二醇（PROH）和蔗糖作为冷冻保护剂以及人血清作为蛋白质支持的效果。测试的特定浓度为 1.26、1.5 和 1.08 mol/l PROH、0.175、0.2、0.224 和 0.3 mol/l 蔗糖以及 20%、30%和 40%人血清在冷冻溶液中和稀释溶液中的正常或升高的蔗糖浓度。来自 13 名年龄在 5-38 岁的患者的卵巢皮质切片使用慢速冷冻-快速解冻进行冷冻保存。使用光和透射电子显微镜进行测试。冷冻损伤主要发生在基质和卵泡细胞中。使用冷冻保护剂浓度最低（1.26 mol/l PROH+0.175 mol/l 蔗糖）的冷冻-解冻方案获得了形态特征的最佳保存，其中使用 30%人血清。
