Zhang Jian-Min, Li Lin-Xia, Liu Xue-Lian, Yang Yi-Xia, Wan Xiao-Ping
Department of Obstetrics and Gynecology, First People's Hospital Affiliated to Shanghai Jiao Tong University, Haining Road 100, Shanghai 200080, China.
J Assist Reprod Genet. 2009 Mar;26(2-3):137-42. doi: 10.1007/s10815-009-9295-9. Epub 2009 Feb 10.
The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues.
Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation.
The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.
Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.
本实验旨在检测不同水平蔗糖对玻璃化冷冻卵巢组织的影响。
将小鼠卵巢组织进行玻璃化冷冻-解冻处理。小鼠被随机分为新鲜对照组和实验组。根据玻璃化溶液中蔗糖浓度的不同，实验组被随机分为I组（0.2M蔗糖）、II组（0.4M蔗糖）、III组（0.8M蔗糖）和IV组（1.6M蔗糖）。在整个卵巢切除和移植期间进行细胞学观察。移植后1个月测量激素水平和卵泡密度。
对照组恢复发情周期前的天数显著少于所有实验组。对照组小鼠血清雌二醇水平和卵巢移植组织的卵泡密度显著高于所有实验组。此外，II组和III组恢复发情周期前的天数少于I组和IV组。II组和III组小鼠血清雌二醇水平和卵巢移植组织的卵泡密度显著高于I组和IV组。然而，II组和III组在恢复发情周期前的天数和血清雌二醇水平方面未观察到差异。II组和III组的卵泡密度也相似。
冷冻保护介质中0.4M或0.8M的蔗糖浓度适合玻璃化冷冻小鼠卵巢组织。
