de Oliveira Carolina Camargo, de Oliveira Simone Martins, Goes Viviane M, Probst Christian M, Krieger Marco Aurelio, Buchi Dorly de Freitas
Departamento de Biologia Celular, Universidade Federal do Paraná, SCB, Curitiba, Paraná, Brazil.
J Cell Biochem. 2008 Jul 1;104(4):1364-77. doi: 10.1002/jcb.21713.
Canova (CA) is a complex homeopathic medication used in diseases where the immune system is depressed. Previous studies demonstrated that it is neither toxic nor mutagenic and activates macrophages. We now evaluate CA effects on cytokine production and gene expression from mice macrophages. The global view of changes in expression of genes with known functions can provide a vivid picture of the way in which cell adapts to a changing environment or a challenge. We found a decrease in IL-2 and IL-4 production and a differential expression in 147 genes from CA group. These genes are mainly involved in transcription/translation, cell structure and dynamics, immune response, cytoprotection, enzymatic process, and receptors/ligands. With gene expression analysis we state that this medication provokes a reaction that involves alterations in gene expression profile mainly in the ones involved with macrophages activation, corroborating the laboratorial research and the clinical data.
卡诺瓦（CA）是一种用于免疫系统功能低下疾病的复方顺势疗法药物。先前的研究表明，它既无毒性也无致突变性，还能激活巨噬细胞。我们现在评估CA对小鼠巨噬细胞细胞因子产生和基因表达的影响。对具有已知功能的基因表达变化的整体观察可以生动地描绘出细胞适应变化环境或挑战的方式。我们发现CA组白细胞介素-2（IL-2）和白细胞介素-4（IL-4）的产生减少，且147个基因存在差异表达。这些基因主要参与转录/翻译、细胞结构与动力学、免疫反应、细胞保护、酶促过程以及受体/配体。通过基因表达分析，我们指出这种药物引发了一种反应，主要涉及与巨噬细胞激活相关基因表达谱的改变，这证实了实验室研究和临床数据。
