Cesar Beatriz, Abud Ana Paula R, de Oliveira Carolina C, Cardoso Francolino, Gremski Waldemiro, Gabardo Juarez, Buchi Dorly de Freitas
Departamento de Biologia Celular, Setor de Ciências Biológicas, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brazil.
Micron. 2008 Jun;39(4):461-70. doi: 10.1016/j.micron.2007.02.005. Epub 2007 Feb 22.
Canova is a Brazilian homeopathic medication with immunomodulatory properties, recommended for patients where the immune system is depressed. Previous studies demonstrated that Canova induces up-regulation in numbers of leukocytes. The bone marrow microenvironment is composed of growth factors, stromal cells, extracellular matrix and progenitor cells that differentiate into mature blood cells. We now report the effect of in vitro administration of the medication on the mononuclear differentiation of the bone marrow cell. Swiss mice femurs were dissected cleaned and the cells of the marrow were flushed. The cells were plated, treated or not, incubated for different times and processed for light, transmission and scanning electron, and confocal microscopy analysis. Bone marrow cells showed an enhanced proliferation in vitro in response to Canova medication and Canova plus M-CSF and an increase was also observed in the numbers of the cell niches and ring-shaped nuclei cells. Confocal and transmission and scanning electron microscopy showed the stages of monocyte maturation, with resting and activated cells. With Canova treatment there was a marked increase in cell size, which is mainly attributable to the augmented cytoplasm, an increase in the number of mitochondria, expansion of the RER and an enlarged Golgi. The response to Canova treatment indicates that it influences mononuclear differentiation and activation of bone marrow progenitor and stromal cells.
卡诺瓦是一种具有免疫调节特性的巴西顺势疗法药物，推荐用于免疫系统低下的患者。先前的研究表明，卡诺瓦可诱导白细胞数量上调。骨髓微环境由生长因子、基质细胞、细胞外基质和分化为成熟血细胞的祖细胞组成。我们现在报告该药物体外给药对骨髓细胞单核分化的影响。解剖并清理瑞士小鼠的股骨，冲洗骨髓细胞。将细胞接种，进行或不进行处理，孵育不同时间，然后进行光镜、透射和扫描电子显微镜以及共聚焦显微镜分析。骨髓细胞在体外对卡诺瓦药物以及卡诺瓦加巨噬细胞集落刺激因子（M-CSF）有增殖增强反应，并且细胞龛和环形核细胞数量也增加。共聚焦、透射和扫描电子显微镜显示了单核细胞成熟的阶段，包括静息细胞和活化细胞。经卡诺瓦处理后，细胞大小显著增加，这主要归因于细胞质增多、线粒体数量增加、粗面内质网扩张和高尔基体增大。对卡诺瓦治疗的反应表明它影响骨髓祖细胞和基质细胞的单核分化及活化。
