Laboratório de Pesquisa em Células Inflamatórias e Neoplásicas Depto de Biologia Celular, Setor de Ciências Biológicas, Federal University of Paraná, Brazil.
BMC Cancer. 2010 Mar 25;10:113. doi: 10.1186/1471-2407-10-113.
Melanoma is the most aggressive form of skin cancer and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have had only marginal success. Previous studies in mice demonstrated that a high diluted complex derived from Calcarea carbonica (M8) stimulated the tumoricidal response of activated lymphocytes against B16F10 melanoma cells in vitro.
Here we describe the in vitro inhibition of invasion and the in vivo anti-metastatic potential after M8 treatment by inhalation in the B16F10 lung metastasis model.
We found that M8 has at least two functions, acting as both an inhibitor of cancer cell adhesion and invasion and as a perlecan expression antagonist, which are strongly correlated with several metastatic, angiogenic and invasive factors in melanoma tumors.
The findings suggest that this medication is a promising non-toxic therapy candidate by improving the immune response against tumor cells or even induce direct dormancy in malignancies.
黑色素瘤是皮肤癌中最具侵袭性的形式，也是全球发病率增长最快的癌症。针对黑色素瘤的化学疗法方法仅取得了些许成效。先前在小鼠身上进行的研究表明，一种源自碳酸钙（M8）的高稀释复合物可刺激激活的淋巴细胞对体外 B16F10 黑色素瘤细胞的杀伤反应。
在这里，我们描述了 M8 通过吸入在 B16F10 肺转移模型中的治疗，对体外侵袭的抑制作用以及体内抗转移潜能。
我们发现 M8 至少具有两种功能，既可以作为癌细胞黏附和侵袭的抑制剂，也可以作为基底膜硫酸乙酰肝素蛋白多糖表达的拮抗剂，这与黑色素瘤肿瘤中的几种转移、血管生成和侵袭因子密切相关。
这些发现表明，这种药物通过改善针对肿瘤细胞的免疫反应，甚至诱导恶性肿瘤直接休眠，是一种很有前途的无毒治疗候选药物。
