鼠白血病病毒的引物结合位点依赖性限制需要TRIM28与HP1结合。
Primer binding site-dependent restriction of murine leukemia virus requires HP1 binding by TRIM28.
作者信息
Wolf Daniel, Cammas Florence, Losson Régine, Goff Stephen P
机构信息
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, HHSC 1310, 701 West 168th Street, New York, NY 10032, USA.
出版信息
J Virol. 2008 May;82(9):4675-9. doi: 10.1128/JVI.02445-07. Epub 2008 Feb 20.
Abstract
TRIM28 is a transcriptional corepressor which is required for primer binding site (PBS)-dependent restriction of murine leukemia virus (MLV) replication in embryonic stem and embryonic carcinoma (EC) cells. PBS-dependent restriction of MLV leads to transcriptional silencing of the integrated provirus and has been shown to correlate with TRIM28-mediated recruitment of HP1 to the silenced loci. Here we show, using a cell line with a point mutation in the HP1 binding domain of TRIM28, that interaction with HP1 is absolutely required for the PBS-dependent restriction of MLV in the F9 EC cell line.
摘要
TRIM28是一种转录共抑制因子，它是胚胎干细胞和胚胎癌细胞（EC）中鼠白血病病毒（MLV）复制的引物结合位点（PBS）依赖性限制所必需的。MLV的PBS依赖性限制导致整合前病毒的转录沉默，并且已证明与TRIM28介导的HP1募集到沉默位点相关。在这里，我们使用在TRIM28的HP1结合域中具有点突变的细胞系表明，在F9 EC细胞系中，与HP1的相互作用对于MLV的PBS依赖性限制是绝对必需的。
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