Fitzgibbon Matthew, Law Wendy, May Damon, Detter Andrea, McIntosh Martin
Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Methods Mol Biol. 2008;428:369-82. doi: 10.1007/978-1-59745-117-8_19.
The analysis of protein mixtures by liquid chromatography-mass spectrometry (LCMS) requires tools for viewing and navigating LC-MS data, locating peptides in LC-MS data, and eliminating low-quality peptides. msInspect, an open source platform, can carry out these steps for single experiments and can align and normalize peptide features in comparative studies with multiple LC-MS runs. In addition, msInspect can analyze quantitative studies with and without isotopic labels to generate peptide arrays.
通过液相色谱-质谱联用（LCMS）对蛋白质混合物进行分析，需要用于查看和浏览LC-MS数据、在LC-MS数据中定位肽段以及去除低质量肽段的工具。msInspect是一个开源平台，它可以对单个实验执行这些步骤，并且在涉及多次LC-MS运行的比较研究中，能够对肽段特征进行比对和归一化处理。此外，msInspect可以分析有无同位素标记的定量研究，以生成肽段阵列。
