Kardaszewicz B, Rogala E, Tendera M, Kardaszewicz P, Jarzab J
Katedry i Kliniki Chorób Wewnetrznych i Alergologii, Zabrzu.
Kardiol Pol. 1991;34(1):21-4.
In 32 patients (pts) with hypertrophic cardiomyopathy (HC), 20 pts with ischaemic heart disease (IHD) and 30 healthy controls, the levels of circulating immune complexes (CIC), immunoglobulins A, G and M, C3c and C4 components of the complement, as well as haemolytic activity of the complement were measured. CIC were assessed using two different methods: a) precipitation with 3% polyethylene glycol with subsequent spectrophotometric measurement of protein content in the precipitate, and b) binding with J-125 labelled staphylococcal +protein ++ A. Pts with HC showed a statistically significant increase in concentration of IgM and the immune complexes (shown with both methods) together with a decrease in C4 and hemolytic activity of the complement. In addition an analysis carried out for each individual patient showed that in some cases an increase in immune complexes concentration was paralleled by a decrease in IgG, C4 and haemolytic activity of the complement. This may suggest that in these pts activation of the complement through the classical pathway can occur. In pts with IHD an increase in immune complexes concentration was demonstrated by precipitation method only. Immune globulines and complement components were within limits for the control group. This suggests that in IHD the complement system is not engaged. Our findings indicate that in HC mechanisms other than those present in myocardial ischaemia must be engaged in inducing changes in the immune system.
在32例肥厚型心肌病（HC）患者、20例缺血性心脏病（IHD）患者和30名健康对照者中，检测了循环免疫复合物（CIC）、免疫球蛋白A、G和M、补体的C3c和C4成分以及补体的溶血活性。使用两种不同方法评估CIC：a）用3%聚乙二醇沉淀，随后用分光光度法测量沉淀物中的蛋白质含量；b）与¹²⁵J标记的葡萄球菌A蛋白结合。HC患者的IgM和免疫复合物浓度（两种方法均显示）有统计学意义的升高，同时C4和补体溶血活性降低。此外，对每位患者进行的分析表明，在某些情况下，免疫复合物浓度升高的同时，IgG、C4和补体溶血活性降低。这可能表明在这些患者中可通过经典途径激活补体。在IHD患者中，仅沉淀法显示免疫复合物浓度升高。免疫球蛋白和补体成分在对照组范围内。这表明在IHD中补体系统未被激活。我们的研究结果表明，在HC中，除心肌缺血外的其他机制必定参与了免疫系统的改变。
