Matsuya Hiroyuki, Kushida Taketoshi, Asada Taku, Umeda Masayuki, Wada Takahiko, Iida Hirokazu
Department of Orthopaedic Surgery, Kansai Medical University, Moriguchi, Osaka, Japan.
Mod Rheumatol. 2008;18(2):132-9. doi: 10.1007/s10165-008-0023-6. Epub 2008 Feb 22.
Osteonecrosis (ON) of the femoral head is one of the most serious complications associated with steroid administration. Here, we treated corticosteroid-induced ON in the rabbit by transplanting mesenchymal cells (MCs). Rabbits were injected once with 20 mg/kg of methylprednisolone (MPSL) and divided into three groups as follows: (1) MPSL alone (no further treatment); (2) MPSL+MCs (7 days after MPSL, MCs [1 x 10(7)/2 ml] were injected into the bone marrow cavity of the femurs); (3) MPSL+saline (7 days after MPSL, saline [2 ml] was injected into the bone marrow cavity of the femurs). Subsequently, the incidence of ON in the femurs 4 weeks after MPSL alone and MPSL+saline was 80 and 68.4%, respectively. In contrast, no ON was recorded in rabbits treated with MPSL+MCs. Vascular endothelial growth factor (VEGF) staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end-labeling (TUNEL) staining was more marked in the MPSL alone and MPSL+saline groups than in the MPSL+MCs rabbits. The percentages of cells in the G1 phase in the MPSL+MCs group were significantly lower than in the other two groups. These findings suggest that the injection of autologous MCs into the femur could prevent corticosteroid-induced ON in patients treated with high-dose short-term steroid medication.
股骨头坏死（ON）是与类固醇给药相关的最严重并发症之一。在此，我们通过移植间充质细胞（MCs）治疗兔的皮质类固醇诱导的ON。兔一次性注射20mg/kg甲基泼尼松龙（MPSL），并分为以下三组：（1）单独使用MPSL（不再进一步治疗）；（2）MPSL+MCs（MPSL给药7天后，将MCs[1×10(7)/2ml]注入股骨骨髓腔）；（3）MPSL+生理盐水（MPSL给药7天后，将生理盐水[2ml]注入股骨骨髓腔）。随后，单独使用MPSL和MPSL+生理盐水治疗4周后股骨ON的发生率分别为80%和68.4%。相比之下，MPSL+MCs治疗的兔未记录到ON。单独使用MPSL组和MPSL+生理盐水组的血管内皮生长因子（VEGF）染色和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸（dUTP）缺口末端标记（TUNEL）染色比MPSL+MCs兔更明显。MPSL+MCs组G1期细胞百分比显著低于其他两组。这些发现表明，向股骨注射自体MCs可预防高剂量短期类固醇药物治疗患者的皮质类固醇诱导的ON。
