Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Acta Orthop. 2010 Feb;81(1):154-60. doi: 10.3109/17453671003587101.
Prevention of osteonecrosis after corticosteroid administration would be important. We examined the potential of vitamin E (alpha-tocopherol) to reduce the incidence of corticosteroid-induced osteonecrosis in an animal model.
Japanese white rabbits were divided into 2 groups; the control group was fed a normal diet and the experimental group was fed alpha-tocopherol-supplemented diet in which alpha-tocopherol (600 mg/kg diet) was added to the normal diet. To induce osteonecrosis, high-dose methylprednisolone acetate (MPSL) (20 mg/kg body weight) was injected once into the right gluteus medius muscle of all rabbits. 4 weeks after the injection of MPSL, the presence or absence of osteonecrosis of bilateral femurs was examined histopathologically. The tocopherol/cholesterol ratios were calculated. The plasma levels of thiobarbituric acid-reactive substances (TBARS) were measured.
Alpha-tocopherol-supplemented diet reduced the incidence of osteonecrosis, which developed in 14 of 20 rabbits in the control group and 5 of 21 rabbits in the experimental group (p = 0.004). The tocopherol/cholesterol ratio was higher in the experimental group than in the control group after the alpha-tocopherol administration. The plasma TBARS level was lower in the experimental group than in the control group at 4 weeks after the MPSL administration.
Our findings may offer a new approach for the prevention of corticosteroid-induced osteonecrosis.
预防皮质类固醇给药后发生骨坏死非常重要。我们在动物模型中研究了维生素 E（α-生育酚）降低皮质类固醇诱导性骨坏死发生率的潜力。
日本大白兔分为 2 组；对照组给予正常饮食，实验组给予添加α-生育酚的饮食，即正常饮食中添加α-生育酚（600mg/kg 饮食）。为了诱导骨坏死，所有兔子的右侧臀中肌单次注射高剂量醋酸甲泼尼龙（MPSL）（20mg/kg 体重）。在 MPSL 注射后 4 周，通过组织病理学检查双侧股骨是否存在骨坏死。计算生育酚/胆固醇比值。测量丙二醛反应性物质（TBARS）的血浆水平。
α-生育酚补充饮食降低了骨坏死的发生率，对照组 20 只兔子中有 14 只和实验组 21 只兔子中有 5 只发生骨坏死（p=0.004）。α-生育酚给药后，实验组的生育酚/胆固醇比值高于对照组。MPSL 给药后 4 周，实验组的血浆 TBARS 水平低于对照组。
我们的研究结果可能为预防皮质类固醇诱导性骨坏死提供了一种新方法。
