Zheng Ping, Zhang Junjian, Liu Hanxing, Xu Xiaojuan, Zhang Xiaolian
Department of Neurology, Zhongnan Hospital, Wuhan University, Wuhan City, China.
Curr Neurovasc Res. 2008 Feb;5(1):13-20. doi: 10.2174/156720208783565636.
The current study investigated whether chronic cerebral hypoperfusion produced by permanent bilateral common carotid artery occlusion (2-vessel occlusion (2-VO)) induced cognitive impairment and whether angelica injections alleviated the impairment. Furthermore, the study examined whether 2-VO altered the expression patterns of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the hippocampus of rats and whether angelica injections attenuated the alteration. Rats were divided into four groups to receive either 2-VO surgery or sham surgery followed by either angelica injections or saline injections for eight weeks. Spatial learning in Morris water maze and the expression patterns of BDNF and NGF in the hippocampus of all rats were examined. The results showed that 2-VO significantly impaired spatial learning and memory, and angelica injections significantly reversed the learning and memory impairment. Furthermore, 2-VO resulted in significantly decreased BDNF protein, NGF protein, and NGF mRNA expression in the hippocampus. Angelica injections significantly attenuated the decreased expression. Moreover, spatial learning in Morris water maze was positively correlated to the expression of BDNF and NGF in the hippocampus. Thus, angelica injections might alleviate cognitive impairment during chronic cerebral hypoperfusion through BDNF and NGF.
本研究调查了永久性双侧颈总动脉闭塞（双血管闭塞，2-VO）所致的慢性脑灌注不足是否会引起认知障碍，以及当归注射液是否能减轻这种障碍。此外，该研究还检测了2-VO是否改变大鼠海马中脑源性神经营养因子（BDNF）和神经生长因子（NGF）的表达模式，以及当归注射液是否能减弱这种改变。将大鼠分为四组，分别接受2-VO手术或假手术，随后进行为期八周的当归注射液或生理盐水注射。检测所有大鼠在莫里斯水迷宫中的空间学习能力以及海马中BDNF和NGF的表达模式。结果显示，2-VO显著损害空间学习和记忆能力，而当归注射液能显著逆转这种学习和记忆障碍。此外，2-VO导致海马中BDNF蛋白、NGF蛋白和NGF mRNA表达显著降低。当归注射液能显著减轻这种表达降低。而且，莫里斯水迷宫中的空间学习与海马中BDNF和NGF的表达呈正相关。因此，当归注射液可能通过BDNF和NGF减轻慢性脑灌注不足期间的认知障碍。
