Kola I, Wilton L
Centre for Early Human Development, Monash University, Clayton, Victoria, Australia.
Mol Reprod Dev. 1991 May;29(1):16-21. doi: 10.1002/mrd.1080290104.
This paper describes an efficient technique for the production of metaphase spreads from single blastomeres biopsied from four-cell preimplantation mouse embryos. The karyotype obtained by chromosomal analysis of single biopsied cells is shown to be fully predictive of subsequent fetal karyotype. The data in this study also demonstrate that the entire process of embryo biopsy and karyotypic analysis of biopsied blastomeres does not adversely affect the ability of biopsied embryos to form fetuses after transfer into pseudopregnant recipients. This study has potential clinical relevance in that it demonstrates that chromosomally defective embryos can be accurately identified before implantation. In addition, the techniques developed in this study may facilitate more efficient procedures for the genesis of animal models for human disorders such as Down syndrome and Alzheimers disease.
本文描述了一种高效技术，用于从四细胞期植入前小鼠胚胎活检获得的单个卵裂球中制备中期染色体铺展。通过对单个活检细胞进行染色体分析获得的核型被证明能够完全预测随后的胎儿核型。本研究中的数据还表明，胚胎活检及对活检卵裂球进行核型分析的整个过程，不会对活检胚胎移植到假孕受体后形成胎儿的能力产生不利影响。这项研究具有潜在的临床意义，因为它表明染色体缺陷胚胎在植入前就能被准确识别。此外，本研究中开发的技术可能有助于更高效地建立人类疾病（如下唐氏综合征和阿尔茨海默病）动物模型的程序。
