Wang Qiang, Liang Xiaoping, Liu Zhao, Zhang Qizhi, Carney Paul, Jiang Huabei
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.
Med Phys. 2008 Jan;35(1):216-24. doi: 10.1118/1.2818736.
Diffuse optical tomography (DOT) is a promising functional imaging modality due to its ability to provide quantitative and dynamic tomographic imaging of brain functions. This pilot study was conducted to demonstrate that DOT can be used to visualize the changes in local hemodynamics during seizures. The focal seizure was induced by microinjection of 10 microl of 1.9 mM GABAA antagonist bicuculline methiodide (BMI) into the left parietal neocortex of male Harlen Sprague-Dawley rats, which was imaged by a multispectral continuous-wave DOT system. Functional images were obtained by our finite element based image reconstruction algorithm. A series of dynamic 2D images were obtained to delineate the time course of concentration changes of oxyhaemoglobin, deoxyhaemoglobin, and total hemoglobin in the rat brain during seizure onset. The BMI induced epileptic foci were localized and observed over time from the images obtained. Our results suggest that diffuse optical tomography may be a promising modality for epilepsy imaging due to its ability to localize epileptic foci as well as its potential to map the functional activity in the area of human cerebral cortex in planning of epilepsy surgery.
扩散光学断层扫描（DOT）是一种很有前景的功能成像方式，因为它能够对脑功能进行定量和动态断层成像。这项初步研究旨在证明DOT可用于可视化癫痫发作期间局部血流动力学的变化。通过向雄性哈伦·斯普拉格-道利大鼠的左顶叶新皮层微量注射10微升1.9 mM的GABAA拮抗剂甲基荷包牡丹碱（BMI）诱发局灶性癫痫发作，并用多光谱连续波DOT系统对其进行成像。功能图像通过我们基于有限元的图像重建算法获得。获得了一系列动态二维图像，以描绘癫痫发作开始时大鼠大脑中氧合血红蛋白、脱氧血红蛋白和总血红蛋白浓度变化的时间进程。从获得的图像中对BMI诱发的癫痫病灶进行定位并随时间观察。我们的结果表明，扩散光学断层扫描可能是一种很有前景的癫痫成像方式，因为它有能力定位癫痫病灶，以及在癫痫手术规划中绘制人类大脑皮层区域功能活动图的潜力。
