新型大肠杆菌噬菌体phiEco32的基因组和蛋白质组分析
Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.
作者信息
Savalia Dhruti, Westblade Lars F, Goel Manisha, Florens Laurence, Kemp Priscilla, Akulenko Natalja, Pavlova Olga, Padovan Julio C, Chait Brian T, Washburn Michael P, Ackermann Hans-W, Mushegian Arcady, Gabisonia Tarasii, Molineux Ian, Severinov Konstantin
机构信息
Waksman Institute for Microbiology, Piscataway, NJ 08854, USA.
出版信息
J Mol Biol. 2008 Mar 28;377(3):774-89. doi: 10.1016/j.jmb.2007.12.077. Epub 2008 Jan 11.
Abstract
A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.
摘要
在一项大规模筛选可能用于治疗奶牛乳腺炎的噬菌体的研究中，分离出了一种新型的感染大肠杆菌的噬菌体。对这种名为phiEco32的噬菌体的77554碱基对基因组进行了测序和注释，并通过电子显微镜和蛋白质组学对其病毒粒子进行了表征。鉴定出了两种与宿主RNA聚合酶相互作用的phiEco32编码蛋白。其中一种是ECF家族的σ因子，可能负责某些病毒基因的转录。另一种RNA聚合酶结合蛋白是一种新型转录抑制剂，其作用机制尚待确定。
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