Cui Jiaqi, Zhong Wenshi, Liu Wenhua, Zhang Can, Zou Ling, Ren Huiying
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
Virus Genes. 2023 Apr;59(2):290-300. doi: 10.1007/s11262-022-01964-y. Epub 2023 Jan 6.
A lysogenic phage vB_EcoP_DE5 (hereafter designated DE5) was isolated from donkey-derived Escherichia coli. The bacteriophage was examined by transmission electron microscopy, and the result showed that DE5 belonged to the genus Kuravirus. DE5 was sensitive to changes in temperature and pH, and it could maintain its activity at pH 7 and below 60 ℃. The whole genome sequencing revealed that DE5 had a double-stranded DNA genome of 77, 305 bp with 42.09% G+C content. A total of 126 open reading frames (ORFs) were identified, including functional genes related to phage integration, DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. One phage integrase gene, one autotransporter adhesin gene, and one tRNA gene were predicted in the whole genome, and no genes associated with drug resistance were identified. The phage DE5 integrase contained 187 amino acids and belonged to the small serine recombinase family. BLASTn analysis revealed that phage DE5 had a high-sequence identity (96%) with E. coli phage SU10. Phylogenetic analysis showed that phage DE5 was a member of the genus Kuravirus. The whole genome sequencing of lysogenic phage DE5 enhanced our understanding of lysogenic phages and their therapeutic applications.
从源自驴的大肠杆菌中分离出一种溶原性噬菌体vB_EcoP_DE5（以下简称DE5）。通过透射电子显微镜对该噬菌体进行了检测，结果表明DE5属于Kuravirus属。DE5对温度和pH值的变化敏感，在pH 7及60℃以下能保持其活性。全基因组测序显示，DE5具有一个77305 bp的双链DNA基因组，G+C含量为42.09%。共鉴定出126个开放阅读框（ORF），包括与噬菌体整合、DNA复制与修饰、转录调控、结构和包装蛋白以及宿主细胞裂解相关的功能基因。在全基因组中预测到一个噬菌体整合酶基因、一个自转运黏附素基因和一个tRNA基因，未发现与耐药性相关的基因。噬菌体DE5整合酶含有187个氨基酸，属于小丝氨酸重组酶家族。BLASTn分析显示，噬菌体DE5与大肠杆菌噬菌体SU10具有高度的序列同一性（96%）。系统发育分析表明，噬菌体DE5是Kuravirus属的成员。溶原性噬菌体DE5的全基因组测序加深了我们对溶原性噬菌体及其治疗应用的理解。
