Daar I, Nebreda A R, Yew N, Sass P, Paules R, Santos E, Wigler M, Vande Woude G F
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21701.
Science. 1991 Jul 5;253(5015):74-6. doi: 10.1126/science.1829549.
The endogenous mos proto-oncogene product (Mos) is required for meiotic maturation. In Xenopus oocytes, the ras oncogene product (Ras) can induce meiotic maturation and high levels of M-phase--promoting factor (MPF) independent of endogenous Mos, indicating that a parallel pathway to metaphase exists. In addition, Ras, like Mos and cytostatic factor, can arrest Xenopus embryonic cell cleavage in mitosis and maintain high levels of MPF. Thus, in the Xenopus oocyte and embryo systems Ras functions in the M phase of the cell cycle. The embryonic cleavage arrest assay is a rapid and sensitive test for Ras function.
内源性mos原癌基因产物（Mos）是减数分裂成熟所必需的。在非洲爪蟾卵母细胞中，ras癌基因产物（Ras）可诱导减数分裂成熟并产生高水平的促M期因子（MPF），且不依赖内源性Mos，这表明存在一条通向中期的平行途径。此外，与Mos和细胞静止因子一样，Ras可使非洲爪蟾胚胎细胞的有丝分裂停留在分裂期并维持MPF的高水平。因此，在非洲爪蟾卵母细胞和胚胎系统中，Ras在细胞周期的M期发挥作用。胚胎分裂停滞试验是一种快速且灵敏的Ras功能检测方法。
