Cheng X J, Huang M Y
Department of Parasisology, Faculty of Basic Medical Sciences, Shanahai Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1991;9(1):17-20.
Using agarose gel electrophoresis, the electrophoretic patterns of four enzymes: malate NADP oxidoreductase (malic enzyme) (ME), glucosephosphate isomerase (GPI), phosphoglucomutase (PGM) and hexokinase (HK), were compared and analysed for five strains of Entamoeba histolytica, which had been obtained from patients with acute amebic dysentery or asymptomatic cyst carrier in Beijing, Tianjin and Fujian Province. The results showed that the electrophoretic patterns of all the five strains of E. histolytica belong to pathogenic zymodeme, zymodeme XIV, which is suggested to be the main pathogenic zymodeme in our country.
采用琼脂糖凝胶电泳法,对从北京、天津和福建省急性阿米巴痢疾患者或无症状包囊携带者分离得到的5株溶组织内阿米巴的4种酶,即苹果酸NADP氧化还原酶(苹果酸酶)(ME)、磷酸葡萄糖异构酶(GPI)、磷酸葡萄糖变位酶(PGM)和己糖激酶(HK)的电泳图谱进行了比较和分析。结果显示,所有5株溶组织内阿米巴的电泳图谱均属于致病酶型,即酶型XIV,提示该酶型可能是我国主要的致病酶型。