Haque R, Hall A, Tzipori S
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Ann Trop Med Parasitol. 1990 Dec;84(6):629-32. doi: 10.1080/00034983.1990.11812519.
Stool samples containing Entamoeba histolytica were obtained from two sources: an urban hospital and an urban slum. Using the enzyme patterns of three enzymes (E.C.2.7.1.1 hexokinase, E.C.5.3.1.9 glucose phosphate isomerase and E.C.2.7.5.1 phosphoglucomutase) stained after cellulose acetate electrophoresis, four zymodemes (I, II, XIV and XVI) were identified in 71 isolates. Zymodemes considered to be pathogenic (II and XIV) were identified from 31 of 34 isolates from the urban hospital, and these zymodemes were strongly associated with dysentery and trophozoites containing ingested red blood corpuscles. Blood visible to the naked eye was more commonly seen in stools from which zymodeme XIV was isolated than in those containing zymodeme II (P less than 0.001). Zymodemes considered to be non-pathogenic (I and XVI) were identified in 34 of 37 isolates from slum dwellers and were not associated with blood in the stools.
一家城市医院和一个城市贫民窟。利用醋酸纤维素电泳后染色的三种酶（E.C.2.7.1.1己糖激酶、E.C.5.3.1.9葡萄糖磷酸异构酶和E.C.2.7.5.1磷酸葡萄糖变位酶）的酶谱，在71株分离物中鉴定出四种酶型（I、II、XIV和XVI）。从城市医院的34株分离物中的31株中鉴定出被认为具有致病性的酶型（II和XIV），这些酶型与痢疾以及含有摄入红细胞的滋养体密切相关。与分离出酶型II的粪便相比，在分离出酶型XIV的粪便中肉眼可见的血液更为常见（P小于0.001）。在贫民窟居民的37株分离物中的34株中鉴定出被认为无致病性的酶型（I和XVI），且这些酶型与粪便中的血液无关。
