Abong'o B O, Momba M N B
Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.
J Appl Microbiol. 2008 Aug;105(2):424-31. doi: 10.1111/j.1365-2672.2008.03756.x. Epub 2008 Feb 19.
The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea.
Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25.5% of water, 21.7% of vegetables as well as 56.5% and 43.5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10.3%, 8.6% and 7.8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliC(H7), rfbE(O157) and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12.2% and 8.8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively.
Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients.
Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.
本研究调查了从水、肉类及肉制品、蔬菜以及确诊和未确诊的腹泻人类免疫缺陷病毒/获得性免疫缺陷综合征（HIV/AIDS）患者粪便中分离出的大肠杆菌O157:H7的流行情况及其分子相关性。
采用基于培养的方法和聚合酶链反应技术鉴定大肠杆菌O157:H7。分别有35%的肉制品、25.5%的水、21.7%的蔬菜以及56.5%和43.5%的确诊和未确诊HIV/AIDS患者粪便被推测为大肠杆菌O157阳性。分子检测结果表明，所检测的蔬菜、水和肉制品中分别有10.3%、8.6%和7.8%携带大肠杆菌O157:H7，其fliC(H7)、rfbE(O157)和eaeA基因座与分别从12.2%的确诊HIV/AIDS患者粪便和8.8%的未确诊HIV/AIDS患者粪便中分离出的部分大肠杆菌O157:H7的基因具有同源性。
水、肉类及肉制品和蔬菜是大肠杆菌O157:H7的潜在来源，这些大肠杆菌有可能导致人类尤其是HIV/AIDS患者腹泻。
应格外小心确保HIV/AIDS患者饮用的水和食用的食物安全，因为受污染的水和食物会导致这些患者发生继发感染。
